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Johns Hopkins HealthCare deep sequencing and microarray core facility
Deep Sequencing And Microarray Core Facility, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deep+sequencing+and+microarray+core/deep+sequencing+and+microarray+core/pm39341835-495-13-6
Average 90 stars, based on 1 article reviews
deep sequencing and microarray core facility - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Sequencing:

Article Title: Selection and Application of Tissue microRNAs for Nonendoscopic Diagnosis of Barrett's Esophagus.
Article Snippet: .. MRC Cancer Unit, Hutchison-MRC Research Centre, University of Cambridge, Cambridge, UK; Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Rockville, Maryland; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK; Johns Hopkins Medical Institutions Deep Sequencing and Microarray Core, Baltimore, Maryland; and Cambridge University Hospitals NHS Trust, Cambridge UK ..

Article Title: Nanoparticle-based targeting of microglia improves the neural regeneration enhancing effects of immunosuppression in the zebrafish retina.
Article Snippet: Dissected eyes were placed into Trizol (ThermoFisher Scientific, 15596018) and RNA was extracted using the RNeasy Micro Kit (Qiagen, 74004). .. Samples were then sent to the Deep Sequencing and Microarray Core (Johns Hopkins University) for library preparations and sequencing. .. Samples were processed using the ClonTech SMARTer Ultra Low Input RNA-v4 (Takara 634440) kit, following the manufacturer’s instructions.

Article Title: PARP1 depletion induces RIG-I-dependent signaling in human cancer cells.
Article Snippet: Total RNAs were isolated from cells in culture using RNAeasy mini kit and on column DNase I treatment following manufacturers instruction (Qiagen, Germantown, MD). .. One microgram of total RNA from each sample were submitted to the Johns Hopkins University Deep Sequencing and Microarray Core for sequencing and analyses. .. Briefly, libraries are prepared using Illumina TruSeq stranded total RNA sample preparation kit following the manufacturer’s recommended procedure.

Article Title: Complete Genome Sequence of Strain AC001, a Novel Cultured Member of the Human Oral Microbiome from the Candidate Phylum Saccharibacteria (TM7)
Article Snippet: .. Library preparation and DNA sequencing were performed at the Johns Hopkins Deep Sequencing and Microarray Core. .. Genomic DNA was sheared to 10 to 20 kb using a Covaris g-TUBE and purified using AMPure XP beads (Agencourt Bioscience).

Article Title: A combination treatment based on drug repurposing demonstrates mutation-agnostic efficacy in pre-clinical retinopathy models.
Article Snippet: On-column DNAase digestion (Qiagen RNase-Free DNase; cat # 79254) was used to remove any genomic DNA contamination from the sample. .. RNA samples were sent to the Deep Sequencing and Microarray Core (Johns Hopkins University, USA) for quality control, library preparation, and sequencing. .. Briefly, polyadenylated RNA was selected from the total RNA samples using OligodT conjugated magnetic beads and prepared for sequencing according to the Illumina TruSeq RNA Sample Preparation Kit v2 (# RS-1222001, Illumina).

Article Title: CD44 deletion leading to attenuation of experimental autoimmune encephalomyelitis results from alterations in gut microbiome and short-chain fatty acids
Article Snippet: .. We want to thank H. Hao, Deep Sequencing and Microarray Core, The Johns Hopkins University School of Medicine, Baltimore, MD, USA, for his help. ..

Article Title: Complete Genome Sequence of Strain BB001, a Novel Epibiont Bacterium from the Candidate Phylum Saccharibacteria (TM7)
Article Snippet: Genomic DNA was extracted using the Epicentre MasterPure kit (Lucigen). .. Library preparation and sequencing were completed at Johns Hopkins Deep Sequencing and Microarray Core. .. Genomic DNA was sheared to 10- to 20-kb sizes using a Covaris g-TUBE and was purified by AMPure-XP beads (Agencourt Bioscience).

Article Title: Distinct and common expression of receptors for inflammatory mediators in vagal nodose versus jugular capsaicin-sensitive/TRPV1-positive neurons detected by low input RNA sequencing
Article Snippet: .. Cells from capsaicin response based isolation (capsaicin-responsive jugular and nodose neurons) were processed at the Johns Hopkins Deep Sequencing and Microarray Core. .. Cells from TRPV1 expression based isolation (Td-tomato-positive jugular and nodose neurons, and Td-tomato-negative nodose neurons) were processed at MedImmune Deep Sequencing and Microarray Core independently following the same protocol.

Microarray:

Article Title: Selection and Application of Tissue microRNAs for Nonendoscopic Diagnosis of Barrett's Esophagus.
Article Snippet: .. MRC Cancer Unit, Hutchison-MRC Research Centre, University of Cambridge, Cambridge, UK; Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Rockville, Maryland; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK; Johns Hopkins Medical Institutions Deep Sequencing and Microarray Core, Baltimore, Maryland; and Cambridge University Hospitals NHS Trust, Cambridge UK ..

Article Title: Nanoparticle-based targeting of microglia improves the neural regeneration enhancing effects of immunosuppression in the zebrafish retina.
Article Snippet: Dissected eyes were placed into Trizol (ThermoFisher Scientific, 15596018) and RNA was extracted using the RNeasy Micro Kit (Qiagen, 74004). .. Samples were then sent to the Deep Sequencing and Microarray Core (Johns Hopkins University) for library preparations and sequencing. .. Samples were processed using the ClonTech SMARTer Ultra Low Input RNA-v4 (Takara 634440) kit, following the manufacturer’s instructions.

Article Title: PARP1 depletion induces RIG-I-dependent signaling in human cancer cells.
Article Snippet: Total RNAs were isolated from cells in culture using RNAeasy mini kit and on column DNase I treatment following manufacturers instruction (Qiagen, Germantown, MD). .. One microgram of total RNA from each sample were submitted to the Johns Hopkins University Deep Sequencing and Microarray Core for sequencing and analyses. .. Briefly, libraries are prepared using Illumina TruSeq stranded total RNA sample preparation kit following the manufacturer’s recommended procedure.

Article Title: Complete Genome Sequence of Strain AC001, a Novel Cultured Member of the Human Oral Microbiome from the Candidate Phylum Saccharibacteria (TM7)
Article Snippet: .. Library preparation and DNA sequencing were performed at the Johns Hopkins Deep Sequencing and Microarray Core. .. Genomic DNA was sheared to 10 to 20 kb using a Covaris g-TUBE and purified using AMPure XP beads (Agencourt Bioscience).

Article Title: A combination treatment based on drug repurposing demonstrates mutation-agnostic efficacy in pre-clinical retinopathy models.
Article Snippet: On-column DNAase digestion (Qiagen RNase-Free DNase; cat # 79254) was used to remove any genomic DNA contamination from the sample. .. RNA samples were sent to the Deep Sequencing and Microarray Core (Johns Hopkins University, USA) for quality control, library preparation, and sequencing. .. Briefly, polyadenylated RNA was selected from the total RNA samples using OligodT conjugated magnetic beads and prepared for sequencing according to the Illumina TruSeq RNA Sample Preparation Kit v2 (# RS-1222001, Illumina).

Article Title: CD44 deletion leading to attenuation of experimental autoimmune encephalomyelitis results from alterations in gut microbiome and short-chain fatty acids
Article Snippet: .. We want to thank H. Hao, Deep Sequencing and Microarray Core, The Johns Hopkins University School of Medicine, Baltimore, MD, USA, for his help. ..

Article Title: Complete Genome Sequence of Strain BB001, a Novel Epibiont Bacterium from the Candidate Phylum Saccharibacteria (TM7)
Article Snippet: Genomic DNA was extracted using the Epicentre MasterPure kit (Lucigen). .. Library preparation and sequencing were completed at Johns Hopkins Deep Sequencing and Microarray Core. .. Genomic DNA was sheared to 10- to 20-kb sizes using a Covaris g-TUBE and was purified by AMPure-XP beads (Agencourt Bioscience).

Article Title: Distinct and common expression of receptors for inflammatory mediators in vagal nodose versus jugular capsaicin-sensitive/TRPV1-positive neurons detected by low input RNA sequencing
Article Snippet: .. Cells from capsaicin response based isolation (capsaicin-responsive jugular and nodose neurons) were processed at the Johns Hopkins Deep Sequencing and Microarray Core. .. Cells from TRPV1 expression based isolation (Td-tomato-positive jugular and nodose neurons, and Td-tomato-negative nodose neurons) were processed at MedImmune Deep Sequencing and Microarray Core independently following the same protocol.

DNA Sequencing:

Article Title: Complete Genome Sequence of Strain AC001, a Novel Cultured Member of the Human Oral Microbiome from the Candidate Phylum Saccharibacteria (TM7)
Article Snippet: .. Library preparation and DNA sequencing were performed at the Johns Hopkins Deep Sequencing and Microarray Core. .. Genomic DNA was sheared to 10 to 20 kb using a Covaris g-TUBE and purified using AMPure XP beads (Agencourt Bioscience).

Control:

Article Title: A combination treatment based on drug repurposing demonstrates mutation-agnostic efficacy in pre-clinical retinopathy models.
Article Snippet: On-column DNAase digestion (Qiagen RNase-Free DNase; cat # 79254) was used to remove any genomic DNA contamination from the sample. .. RNA samples were sent to the Deep Sequencing and Microarray Core (Johns Hopkins University, USA) for quality control, library preparation, and sequencing. .. Briefly, polyadenylated RNA was selected from the total RNA samples using OligodT conjugated magnetic beads and prepared for sequencing according to the Illumina TruSeq RNA Sample Preparation Kit v2 (# RS-1222001, Illumina).

Isolation:

Article Title: Distinct and common expression of receptors for inflammatory mediators in vagal nodose versus jugular capsaicin-sensitive/TRPV1-positive neurons detected by low input RNA sequencing
Article Snippet: .. Cells from capsaicin response based isolation (capsaicin-responsive jugular and nodose neurons) were processed at the Johns Hopkins Deep Sequencing and Microarray Core. .. Cells from TRPV1 expression based isolation (Td-tomato-positive jugular and nodose neurons, and Td-tomato-negative nodose neurons) were processed at MedImmune Deep Sequencing and Microarray Core independently following the same protocol.



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